The lab is seeking a highly motivated and enthusiastic individual to perform basic and translational research in the area of pediatric lung diseases including asthma, infection and bronchopulmonary dysplasia. The successful applicant is expected to perform research experiments, maintain records of experiments and analyze data, and assist in preparation of grants and manuscripts.
1. Perform research experiments independently or semi-independently in accordance with protocols approved by the principal investigator. Such experiments may involve the use of isolated human or mouse primary cell culture lines or laboratory animals. These may require evening and/or weekend duties.
2. Collect and process human lung tissue, blood and other biospecimens as needed. Isolate cells from human tissue specimens or blood for establishment of cell culture and use for research studies. Such activities may be performed independently and may require evening and weekend hours.
3. Maintain mouse husbandry and perform animal experiments.
4. Perform standard molecular, biochemical, and imaging techniques (qRT PCR, Western blot, ELISA, IHC, etc.). Perform additional duties as appropiate
5. Collects and controls experimental data and relevant subject information; maintains accurate daily logs of experiments including methods, results, and conclusions; and maintains organized data files.
6. Interprets the results of data analyses with input from the principal investigator.
7. Assist the principal investigator in the preparation of scientific publications and grant proposals and establishing, optimizing, and refining standard laboratory techniques.
8. Orders supplies needed to conduct research; maintains inventory of equipment and supplies; and assures that research equipment is in good working order. Conduct experiments within budgetary constraints.
9. Instructs other laboratory workers and students in standard methods, practices, procedures, and equipment operations.
10. Maintains a clean working area and performs general laboratory maintenance. Adheres to proper safety techniques and procedures.
The applicant will report to Dr. Rodney Britt Jr, Principle Investigator, Center for Perinatal Research, Nationwide Children’s Hospital.
KNOWLEDGE , SKILLS AND ABILITIES REQUIRED
Bachelor’s Degree in a relevant field of science from an accredited college.
Six months to two years of relevant laboratory experience is preferred.
Coursework in biochemistry, molecular biology, physiology, genetics, statistics, and biology-related research methodology is desirable.
MINIMUM PHYSICAL REQUIREMENTS Percent of time spent on: 50% Preparing maintaining cell cultures, performing experiments for cell and animal studies 25% Interpreting results of data analyses with PI 10% Miscellaneous paperwork associated with research 10% Ordering supplies 05% Instructing other lab personnel Minimum physical requirements: 100% Kneeling / reaching 100% Finger dexterity 100% Talking / hearing / seeing 33% Lifting / carrying weight 0-20 pounds for 1-5 minutes
The above list of duties is intended to describe the general nature and level of work performed by individuals assigned to this classification. It is not to be construed as an exhaustive list of duties performed by the individual so classified, nor is it intended to limit or modify the right of any supervisor to assign, direct, and control the work of employees under his/her supervision. EOE M/F/Disability/Vet